MAX IV Laboratory

 

MAXlab300400

The MAX IV Laboratory, the successor of the MAX-lab national laboratory, includes both the operation of the present MAX I, II and III facilities (MAX-lab) as well as the construction of the new MAX IV facility in Lund, Sweden. The new facility will consist of a linac and two storage rings with 1.5 GeV and 3 GeV electron energy. The design of the 3 GeV storage ring uses a multibend achromats approach and it will be the first synchrotron using this new type of lattice. The 3 GeV ring will have exceptionally low emittance which will lead to an unprecedented brilliance for a synchrotron radiation source.

 

BioMAX will be one of the seven initial beamlines at the new facility. It will be a high-throughput macromolecular crystallography (MX) beamline. It will offer high brilliance, tunable (0.5–2.5 Å wavelength), monochromatic X-rays with state-of-the-art performance in terms of low beam-divergence (0.1 mrad), high X-ray flux (~1013 photons/s) and variable beam size (10–100 μm), allowing work with both small crystals and large biomolecular complexes with concomitantly large unit cells. BioMAX is designed to be flexible and serve a broad range of needs for the life science community.

 

At the current MAX II storage ring we operate two beamlines for macromolecular crystallography, I911-2 and -3. The I911-3 beamline is energy tunable in the range between 6.2 – 16.5 keV (0.75 – 2.0 Å). The experimental setup includes a MD2 microdiffractometer with a MK3 mini-kappa, on-axis sample viewing, three-click centring and an Xflash silicon drift diode fluorescence detector. Diffraction data are collected using a Marmosaic 225 detector. The fluorescence detector can be used both for fluorescence scans (scanning the incoming X-ray beam energy over the absorption edge of a particular element) and measuring fluorescence spectra to, for example, quickly identify elements in the crystal. The beamline has a CATS automatic sample changer with a capacity of 90 samples using standard EMBL/ESRF pucks. The CATS sample changer can also be used for screening crystallisation plates for diffracting crystals. An EMBL Grenoble-type humidity control device (HC1) is available on request for optimising the diffraction properties of crystals or test samples at room temperature.

I911-2 is a fixed wavelength beamline (1.04 Å) equipped with a MarDTB goniostat and a Mar165 CCD detector.  A suite of software packages is maintained for smooth data collection and analysis at the beamlines.

In addition, a small user laboratory including a crystallization facility is located within the current facility.

 

More general information on the MAX IV Laboratory can be found at www.maxlab.lu.se, for specific information for BioMAX see https://www.maxlab.lu.se/biomax and for the current MX beamlines https://www.maxlab.lu.se/node/34 .